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Journal: Advanced Science
Article Title: Mucous Permeable Nanoparticle for Inducing Cuproptosis‐Like Death In Broad‐Spectrum Bacteria for Nebulized Treatment of Acute Pneumonia
doi: 10.1002/advs.202408580
Figure Lengend Snippet: Activation of macrophage response by Cu 2 O‐BSO NPs in vitro. a) Typical scatter plots of MH‐S surface markers CD86 (M1 macrophage marker) and CD206 (M2 macrophage marker) detected by flow cytometry. b) Typical images of MH‐S engulfing a bacterium. The green fluorescence represents GFP‐MRSA, the red fluorescence represents MH‐S, and the blue fluorescence represents the nucleus. The stronger the green fluorescence, the stronger the phagocytosis of MH‐S. c,d) Representative SPM photographs and CFU count of MRSA engulfed by MH‐S. e–g) ELISA results indicating levels of cytokines (IL‐1β, IL‐6, and TNF‐α) secreted by MH‐S in different groups.
Article Snippet: Each group of cells was centrifuged, washed, and resuspended in 500 μL of PBS, which contained an
Techniques: Activation Assay, In Vitro, Marker, Flow Cytometry, Fluorescence, Enzyme-linked Immunosorbent Assay